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Vimentin antibody

Cat. No. GTX28545

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC

Reactivity

 Human
Package
7 ml ($399)
See all Vimentin products

APPLICATION

Application Note

Immunohistochemistry (IHC) 1:50-1:100 using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears do not require antigen retrieval.

Calculated MW

54 kDa. ( Note )

Positive Control

Sarcomas, melanomas, Hela cells

PROPERTIES

Form

Liquid

Buffer

10mM PO₄ pH7.4, 200mM NaCl, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

10 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human Vimentin

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm , Cytoplasm, cytoskeleton

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399