GeneTex
United States (US)

Vimentin antibody

Cat No. GTX28545

Host Rabbit
Clonality Polyclonal
Application IHC
Reactivity Human
APPLICATION

Application Note

Immunohistochemistry (IHC) 1:50-1:100 using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears do not require antigen retrieval.

Calculated MW

54 kDa. ( Note )

Positive Control

Sarcomas, melanomas, Hela cells
PROPERTIES

Form

Liquid

Buffer

10mM PO4, 200mM NaCl, pH 7.4, containing 1% BSA and 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

10μg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human Vimentin

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vimentin Antibody , VIM Antibody

Cellular Localization

Cytoplasmic

Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Database

Research Area

Package List Price ($)
$ 319