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Vimentin antibody

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30668
Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30668
Anti-Vimentin antibody used in Western Blot (WB). GTX30668
Anti-Vimentin antibody used in IHC (Frozen sections) (IHC-Fr). GTX30668

Cat. No. GTX30668

Reference 1

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Bovine, Dog, Chicken, Pig, Horse
Package
100 μl ($599)
See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:10000
ICC/IF 1:5000
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Vimentin purified from E. coli. [Swiss-Prot# P08670]

Purification

Purified IgY

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30668

GTX30668 ICC/IF Image

ICC/IF analysis of 10% formalin-fixed A7 cells using GTX30668 Vimentin antibody.
Permeabilization : 0.1% TritonX-100
Dilution : 1:600 at 4 oC overnight

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30668

GTX30668 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30668 Vimentin antibody.
Green : Primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:500

Anti-Vimentin antibody used in Western Blot (WB). GTX30668

GTX30668 WB Image

WB analysis of rat whole brain tissue (lane 2), HeLa (lane 3), SH-SY5Y (lane 4), HEK293 (lane 5), and NIH-3T3 (lane 6) cell lysates using GTX30668 Vimentin antibody (Red) and MAP2 antibody (Green). MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
Dilution : 1:5000

Anti-Vimentin antibody used in IHC (Frozen sections) (IHC-Fr). GTX30668

GTX30668 IHC-Fr Image

IHC-Fr analysis of 10 um PFA fixed mouse brain acute slices using GTX30668 Vimentin antibody.
Dilution : 1:600

REFERENCE

Application Reference

Yu Q et al. Nat Commun 2023; 14 (1) : 1588 Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 599