GeneTex
United States (US)

Vimentin antibody

GTX30668_232_ICC-IF_18121410_112.jpg
GTX30668_1469_WB_18121410_468.jpg

Cat No. GTX30668

Host Chicken
Clonality Polyclonal
Isotype IgY
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:10000 - 1:20000
ICC/IF 1:5000
IHC-P 1:150
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

5mM Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Vimentin purified from E. coli. [Swiss-Prot# P08670]

Purification

Purified IgY

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vimentin,Vim

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX30668_232_ICC-IF_18121410_112.jpg

GTX30668 ICC/IF Image

ICC/IF analysis of mixed neuron/glia cell cultures using GTX30668 Vimentin antibody. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
Red : GFAP
Green : Vimentin
Blue : DAPI

GTX30668_1469_WB_18121410_468.jpg

GTX30668 WB Image

WB analysis of HEK293 cell lysate using GTX30668 Vimentin antibody.

Package List Price ($)
$ 339