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Vimentin antibody

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50765
Anti-Vimentin antibody used in Western Blot (WB). GTX50765

Cat No. GTX50765

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Monkey
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.452~456(G-Q-V-I-N) derived from human Vimentin.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES
Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50765

GTX50765 ICC/IF Image

ICC/IF analysis of human mesenchymal stem cells using GTX50765 Vimentin antibody.

Anti-Vimentin antibody used in Western Blot (WB). GTX50765

GTX50765 WB Image

WB analysis of extracts from HeLa, COS7, and JK cells using GTX50765 Vimentin antibody.

REFERENCE
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Package List Price ($)
$ 319