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Vimentin antibody

Anti-Vimentin antibody used in Immunohistochemistry (IHC). GTX85471
Anti-Vimentin antibody used in Immunohistochemistry (IHC). GTX85471
Anti-Vimentin antibody used in Western Blot (WB). GTX85471

Cat. No. GTX85471

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:5000
ICC/IF Assay dependent
IHC 1:1000-1:2000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

300 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the vimentin gene product, but are shared between the human (NP_003371, NCBI) and mouse (NP_035831, NCBI) protein sequences.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm , Cytoplasm, cytoskeleton

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody used in Immunohistochemistry (IHC). GTX85471

GTX85471 IHC Image

IHC analysis of adult mouse cerebellum tissue using GTX85471 Vimentin antibody.
Dilution : 1:1000

Anti-Vimentin antibody used in Immunohistochemistry (IHC). GTX85471

GTX85471 IHC Image

IHC analysis of neurosphere cells from an e16 mouse brain using GTX85471 Vimentin antibody.
Dilution : 1:1000

Anti-Vimentin antibody used in Western Blot (WB). GTX85471

GTX85471 WB Image

WB analysis of neurosphere culture lysate using GTX85471 Vimentin antibody.
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399