Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody [GT11512] (GTX633923) diluted at 1:3000.

Vimentin (phospho Ser56) antibody [GT11512] detects Vimentin (phospho Ser56) protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin (phospho Ser56) antibody [GT11512] (GTX633923) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min