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Vimentin (phospho Ser56) antibody [GT11512]

Anti-Vimentin (phospho Ser56) antibody [GT11512] used in Western Blot (WB). GTX633923
Anti-Vimentin (phospho Ser56) antibody [GT11512] used in IHC (Paraffin sections) (IHC-P). GTX633923

Cat No. GTX633923

Host

Mouse

Clonality

Monoclonal

Clone Name

GT11512

Isotype

IgG1

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa(100 ng/ml Nocodazole treatment for 24 hr)

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Vimentin (phospho Ser56). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888395

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Vimentin (phospho Ser56) antibody [GT11512] used in Western Blot (WB). GTX633923

GTX633923 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody [GT11512] (GTX633923) diluted at 1:3000.

Anti-Vimentin (phospho Ser56) antibody [GT11512] used in IHC (Paraffin sections) (IHC-P). GTX633923

GTX633923 IHC-P Image

Vimentin (phospho Ser56) antibody [GT11512] detects Vimentin (phospho Ser56) protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin (phospho Ser56) antibody [GT11512] (GTX633923) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for Vimentin (phospho Ser56) antibody [GT11512] (GTX633923). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Vimentin (phospho Ser56) antibody [GT11512] (GTX633923). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169
Custom Conjugations