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Vimentin (phospho Ser56) antibody

Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607
Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607
Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607

Cat No. GTX132607

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , HeLa , NT2D1 , HeLa (100 ng/ml Nocodazole, 24 hr)

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Vimentin (phospho Ser56). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607

GTX132607 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (GTX132607) diluted at 1:5000.

Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607

GTX132607 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (GTX132607) diluted at 1:1000.

Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607

GTX132607 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (GTX132607) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169