APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5 - 1 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
PC-3 Cell Lysate(GTX23954)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.The immunogen is located within amino acids 50 - 100 of Vinculin.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL
Cellular Localization
Cell membrane,Cytoplasm, cytoskeleton
Background
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX17308 WB Image
WB analysis of 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and rat heart, mouse lung, rat lung, mouse spleen, rat spleen, rabbit spleen, mouse brain, rabbit brain and chicken spleen tissue lysate using GTX17308 Vinculin antibody [7E10]. Working concentration : 1 μg/ml
|
REFERENCE
There are currently no references for Vinculin antibody [7E10] (GTX17308). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Vinculin antibody [7E10] (GTX17308). Be the first to share your experience with this product.