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Vinculin antibody [7E10]

Anti-Vinculin antibody [7E10] used in Western Blot (WB). GTX17308

Cat. No. GTX17308

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7E10

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat, Rabbit, Chicken
Package
100 μg ($429)
See all Vinculin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

PC-3 Cell Lysate(GTX23954)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.The immunogen is located within amino acids 50 - 100 of Vinculin.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Vinculin antibody [7E10] used in Western Blot (WB). GTX17308

GTX17308 WB Image

WB analysis of 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and rat heart, mouse lung, rat lung, mouse spleen, rat spleen, rabbit spleen, mouse brain, rabbit brain and chicken spleen tissue lysate using GTX17308 Vinculin antibody [7E10].
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429