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Vinculin antibody [GT3210]

Anti-Vinculin antibody [GT3210] used in Western Blot (WB). GTX634535
Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535
Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535
Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535
Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535
Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535

Cat No. GTX634535

Host

Mouse

Clonality

Monoclonal

Clone Name

GT3210

Isotype

IgG1

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888464

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Vinculin antibody [GT3210] used in Western Blot (WB). GTX634535

GTX634535 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [GT3210] (GTX634535) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535

GTX634535 IHC-P Image

Vinculin antibody [GT3210] detects Vinculin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535

GTX634535 IHC-P Image

Vinculin antibody [GT3210] detects Vinculin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535

GTX634535 IHC-P Image

Vinculin antibody [GT3210] detects Vinculin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535

GTX634535 IHC-P Image

Vinculin antibody [GT3210] detects Vinculin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vinculin antibody [GT3210] used in IHC (Paraffin sections) (IHC-P). GTX634535

GTX634535 IHC-P Image

Vinculin antibody [GT3210] detects Vinculin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for Vinculin antibody [GT3210] (GTX634535). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Vinculin antibody [GT3210] (GTX634535). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations