APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888464
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL
Cellular Localization
Cell membrane,Cytoplasm, cytoskeleton
Background
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX634535 IHC-P Image
Vinculin antibody [GT3210] detects Vinculin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634535 IHC-P Image
Vinculin antibody [GT3210] detects Vinculin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634535 IHC-P Image
Vinculin antibody [GT3210] detects Vinculin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634535 IHC-P Image
Vinculin antibody [GT3210] detects Vinculin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634535 IHC-P Image
Vinculin antibody [GT3210] detects Vinculin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.Vinculin stained by Vinculin antibody [GT3210] (GTX634535) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634535 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [GT3210] (GTX634535) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634535 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [GT3210] (GTX634535) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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