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Vinculin antibody [N1N3]

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Anti-Vinculin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113294
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Anti-Vinculin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113294
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Anti-Vinculin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113294
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

Cat No. GTX113294

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

NT2D1 , Mouse brain , *HeLa

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732545

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113294

GTX113294 IHC-P Image

Vinculin antibody [N1N3] detects Vinculin protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Vinculin antibody [N1N3] (GTX113294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Vinculin antibody detects Vinculin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113294

GTX113294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Vinculin(GTX113294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113294

GTX113294 ICC/IF Image

Vinculin antibody [N1N3] detects Vinculin protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Vinculin stained by Vinculin antibody [N1N3] (GTX113294) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Vinculin antibody [N1N3] Cat No. GTX113294

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31758038
Date : Anonymous submitted on 21-Jun-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : Raw264.7 cells
Amount used : 20 μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 23-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 30μg
Blocking : 5% non-fat milk, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 9Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169