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Vinculin antibody [N1N3]

Orthogonal
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Anti-Vinculin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113294
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Anti-Vinculin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113294
Anti-Vinculin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113294
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

Cat. No. GTX113294

Reference 8
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Vinculin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732545

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Vinculin antibody detects Vinculin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113294

GTX113294 IHC-P Image

Vinculin antibody [N1N3] detects Vinculin protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Vinculin antibody [N1N3] (GTX113294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113294

GTX113294 ICC/IF Image

Vinculin antibody [N1N3] detects Vinculin protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Vinculin stained by Vinculin antibody [N1N3] (GTX113294) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Vinculin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113294

GTX113294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Vinculin(GTX113294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294

GTX113294 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2019. PMID: 31758038

REFERENCE

Application Reference

Miglietta S et al. Noncoding RNA 2022; 8 (5) MicroRNA and Metabolic Profiling of a Primary Ovarian Neuroendocrine Carcinoma Pulmonary-Type Reveals a High Degree of Similarity with Small Cell Lung Cancer.
D'Angelo L et al. Cell Rep 2021; 35 (3) : 109002 NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate.
Application : WB
Reactivity : Human
Boschetti E et al. Am J Physiol Gastrointest Liver Physiol 2021; 320 (5) : G768-G779 Evidence of enteric angiopathy and neuromuscular hypoxia in patients with mitochondrial neurogastrointestinal encephalomyopathy.
Boschetti E et al. Neurogastroenterol Motil 2019; 31 (8) : e13652 Gut epithelial and vascular barrier abnormalities in patients with chronic intestinal pseudo-obstruction.
Inder S et al. Sci Rep 2019; 9 (1) : 17325 Multiplex profiling identifies clinically relevant signalling proteins in an isogenic prostate cancer model of radioresistance.
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Submit a Reference

REVIEW

Vinculin antibody [N1N3] Cat. No. GTX113294

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Date : Anonymous submitted on 21-Jun-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : Raw264.7 cells
Amount used : 20 μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Vinculin antibody [N1N3] used in Western Blot (WB). GTX113294
Date : Anonymous submitted on 23-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 30μg
Blocking : 5% non-fat milk, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 9Hr
Submit a Review
Application Reference
Miglietta S et al. Noncoding RNA 2022; 8 (5) MicroRNA and Metabolic Profiling of a Primary Ovarian Neuroendocrine Carcinoma Pulmonary-Type Reveals a High Degree of Similarity with Small Cell Lung Cancer.
D'Angelo L et al. Cell Rep 2021; 35 (3):109002 NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate.
Application : WB
Reactivity : Human
Boschetti E et al. Am J Physiol Gastrointest Liver Physiol 2021; 320 (5):G768-G779 Evidence of enteric angiopathy and neuromuscular hypoxia in patients with mitochondrial neurogastrointestinal encephalomyopathy.
Boschetti E et al. Neurogastroenterol Motil 2019; 31 (8):e13652 Gut epithelial and vascular barrier abnormalities in patients with chronic intestinal pseudo-obstruction.
Inder S et al. Sci Rep 2019; 9 (1):17325 Multiplex profiling identifies clinically relevant signalling proteins in an isogenic prostate cancer model of radioresistance.
Haug MF et al. Histochem Cell Biol 2019; (Epub) Differential expression of PKCα and -β in the zebrafish retina.
Application : WB
Reactivity : Human
Ko CC et al. J Biol Chem 2014; Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of Quercetin in its anti-cancer effects in PC-3 cells.
Application : WB
Reactivity : Human
Liou YR et al. PLoS One 2014; 9 (2):e89767 Substrate stiffness regulates filopodial activities in lung cancer cells.
Application : ICC/IF
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169