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Vinculin antibody [N3C1], Internal

Orthogonal
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749
Anti-Vinculin antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109749
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

Cat No. GTX109749

Reference 8
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Vinculin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

PC-12 , Rat2 , Jurkat , Raji , NCI-H929 , Mouse muscle

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952540

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

GTX109749 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

GTX109749 WB Image

Vinculin antibody [N3C1], Internal detects VCL protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000

Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

GTX109749 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000.

Anti-Vinculin antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109749

GTX109749 IHC-P Image

Immunohistochemical analysis of human breast cancerncer, using Vinculin(GTX109749) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

GTX109749 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cunningham S. et al. Cancers 2021; 13 (5) : 1012 FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice.
Application : WB
Reactivity : Mouse
Yang CW et al. Biochem Pharmacol 2020; 180 : 114122 Natural cardenolides suppress coronaviral replication by downregulating JAK1 via a Na+/K+-ATPase independent proteolysis.
Macchi C et al. Am J Pathol 2020; 190 (11) : 2226-2236 Leptin, Resistin, and Proprotein Convertase Subtilisin/Kexin Type 9: The Role of STAT3.
Application : WB
Reactivity : Human
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Chou CW et al. Sci Rep 2019; 9 (1) : 20403 Therapeutic effects of statins against lung adenocarcinoma via p53 mutant-mediated apoptosis.
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REVIEW

Vinculin antibody [N3C1], Internal Cat No. GTX109749

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 31892709
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749
Date : Anonymous submitted on 16-Jun-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : Raw264.7 cells
Amount used : 20 μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749
Date : Anonymous submitted on 29-Oct-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : MO3.13 cells
Amount used : 10 μg
Blocking : Horse serum, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169