GeneTex
United States (US)

Vinculin antibody [N3C1], Internal

GTX109749_40037_WB_19011507_231.jpg
GTX109749_40037_WB_R_19011507_479.jpg
GTX109749_40037_20151105_WB_M_muscle_19011507_655.jpg
GTX109749_40037_IHC_19011507_520.jpg

Cat No. GTX109749

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
See all Vinculin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

PC-12 , Rat2 , Jurkat , Raji , NCI-H929 , Mouse muscle

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CMD1W antibody, CMH15 antibody, MVCL antibody, VCL antibody, metavinculin antibody, vinculin antibody

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]
Show More +

Database

Research Area

DATA IMAGES
GTX109749_40037_WB_19011507_231.jpg

GTX109749 WB Image

Vinculin antibody [N3C1], Internal detects Vinculin protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg H1299 whole cell lysate/extract
D. 30 μg HeLa whole cell lysate/extract
E. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000
GTX109749_40037_WB_R_19011507_479.jpg

GTX109749 WB Image

Vinculin antibody [N3C1], Internal detects VCL protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
5 % SDS-PAGE
Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000
GTX109749_40037_20151105_WB_M_muscle_19011507_655.jpg

GTX109749 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000.
GTX109749_40037_IHC_19011507_520.jpg

GTX109749 IHC-P Image

Immunohistochemical analysis of human breast cancerncer, using Vinculin(GTX109749) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Brandi J et al. J Proteomics 2016; 136:43112 Secretome protein signature of human pancreatic cancer stem-like cells.
Application : WB
Reactivity : Human
Submit a Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169