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Vinculin antibody [N3C1], Internal

Orthogonal
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749
Anti-Vinculin antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109749
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

Cat No. GTX109749

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all Vinculin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

PC-12 , Rat2 , Jurkat , Raji , NCI-H929 , Mouse muscle

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vinculin,CMD1W,CMH15,HEL114,MV,MVCL

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

GTX109749 WB Image

Vinculin antibody [N3C1], Internal detects VCL protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
5 % SDS-PAGE
Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

GTX109749 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000.
Anti-Vinculin antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109749

GTX109749 IHC-P Image

Immunohistochemical analysis of human breast cancerncer, using Vinculin(GTX109749) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

GTX109749 WB Image

Orthogonal

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
REFERENCE

Application Reference

Brandi J et al. J Proteomics 2016; 136 : 43112 Secretome protein signature of human pancreatic cancer stem-like cells.
Application : WB
Reactivity : Human
Submit a Reference
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169