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Vinculin antibody [VCL/2572]

Anti-Vinculin antibody [VCL/2572] used in Western Blot (WB). GTX17827
Anti-Vinculin antibody [VCL/2572] used in IHC (Paraffin sections) (IHC-P). GTX17827
Anti-Vinculin antibody [VCL/2572] used in Protein Array (Protein Array). GTX17827

Cat No. GTX17827

Host

Mouse

Clonality

Monoclonal

Clone Name

VCL/2572

Isotype

IgG1

Application

WB, IHC-P, Protein Array

Reactivity

Human
Package
100 μg ($469)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CMD1W , CMH15 , HEL114 , MV , MVCL , VCL , vinculin , Vinculin

Cellular Localization

Cytoplasm,Cytoskeleton,Cell junction,Adherens junction,Cell membrane,Focal adhesion

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Vinculin antibody [VCL/2572] used in Western Blot (WB). GTX17827

GTX17827 WB Image

WB analysis of U-87 and THP-1 cell lysates using GTX17827 Vinculin antibody [VCL/2572].

Anti-Vinculin antibody [VCL/2572] used in IHC (Paraffin sections) (IHC-P). GTX17827

GTX17827 IHC-P Image

IHC-P analysis of human testis tissue using GTX17827 Vinculin antibody [VCL/2572].

Anti-Vinculin antibody [VCL/2572] used in Protein Array (Protein Array). GTX17827

GTX17827 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Mouse Monoclonal Antibody (VCL/2572). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469