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Vinculin antibody [VIN-11-5]

Anti-Vinculin antibody [VIN-11-5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11193
Anti-Vinculin antibody [VIN-11-5] used in Western Blot (WB). GTX11193

Cat. No. GTX11193

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 VIN-11-5

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Bovine, Chicken, Gerbil
Package
200 μl ($719)
See all Vinculin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:50
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Positive Control

chicken fibroblast

Product Note

The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Chicken

Immunogen

smooth muscle vinculin from chicken gizzard.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_381274

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vinculin

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton

Background

Cytoskeletal filaments are associated with the plasma membrane in areas of cell contact. In particular it has been shown that bundles of actin containing microfilaments are attached to the cytoplasmic faces of the membrane in cell-substrate focal contacts (adhesion plaques) of cultured fibroblasts. Besides actin, which is the major constituent of microfilaments, other actin related proteins are found close to or in the area of microfilament membrane association in contact regions. Vinculin is associated with the cytoplasmic aspect of contact areas close to the membrane. It has been suggested that vinculin is a possible link between ends of the bundles of actin filaments and the plasma membrane. It has also been proposed that vinculin may be involved in the transmembrane induction of actin bundle formation.Monoclonal Anti-Vinculin may be used for the immunocytochemical localization of vinculin using indirect immunofluorescent labeling of cultured fibroblasts or for specific identification of the vinculin band in immunoblotting techniques.

Database

Research Area

DATA IMAGES

Anti-Vinculin antibody [VIN-11-5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11193

GTX11193 ICC/IF Image

ICC/IF analysis of an isolated smooth muscle cell (chicken gizzard)using GTX11193 Vinculin antibody [VIN-11-5] (green) and anti-alpha-Actinin antibody (red).

Anti-Vinculin antibody [VIN-11-5] used in Western Blot (WB). GTX11193

GTX11193 WB Image

WB analysis of chicken gizzard extract using GTX11193 Vinculin antibody [VIN-11-5] at 1:200.

REFERENCE

Application Reference

Miyoshi H et al. Anal Sci 2016; 32 (11) : 1207-1211 A Perturbation Analysis to Understand the Mechanism How Migrating Cells Sense and Respond to a Topography in the Extracellular Environment.
Application : ICC/IF
Reactivity : Gerbil
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 719