GeneTex
United States (US)

Vinculin antibody

GTX32965_20180530_IHC-P_18121410_636.jpg
GTX32965_20180530_WB_18121410_465.jpg

Cat No. GTX32965

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

124 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 767-1066 of human VCL (NP_003364.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vinculin,Cmd1W,Cmh15,Hel114,Mv,Mvcl,Vcl

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32965_20180530_IHC-P_18121410_636.jpg

GTX32965 IHC-P Image

IHC-P analysis of human smooth muscle tissue using GTX32965 Vinculin antibody.
Dilution : 1:100

GTX32965_20180530_WB_18121410_465.jpg

GTX32965 WB Image

WB analysis of various samples using GTX32965 Vinculin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329