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Vinculin (phospho Tyr100) antibody

Orthogonal
Anti-Vinculin (phospho Tyr100) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54517
Anti-Vinculin (phospho Tyr100) antibody used in Western Blot (WB). GTX54517
Anti-Vinculin (phospho Tyr100) antibody used in Western Blot (WB). GTX54517

Cat. No. GTX54517

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Chicken
Package
100 μl ($399)
See all Vinculin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 100. The sequence is conserved in mouse and chicken.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Vinculin (phospho Tyr100) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54517

GTX54517 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54517 Vinculin (phospho Tyr100) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Anti-Vinculin (phospho Tyr100) antibody used in Western Blot (WB). GTX54517

GTX54517 WB Image

Orthogonal

WB analysis of COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA which were either untreated (Lane 1 and 6) or treated with vanadate for 24 hr (Lane 2-5 and 7-10) using GTX54517 Vinculin (phospho Tyr100) antibody (left panel) or Vinculin antibody (right panel).

Anti-Vinculin (phospho Tyr100) antibody used in Western Blot (WB). GTX54517

GTX54517 WB Image

Orthogonal

WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1) and HeLa treated with 100ng/ml of LPS for 20mins (Lane 2) using GTX54517 Vinculin (phospho Tyr100) antibody.
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399