ICC/IF analysis of HeLa cells using GTX54517 Vinculin (phospho Tyr100) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1) and HeLa treated with 100ng/ml of LPS for 20mins (Lane 2) using GTX54517 Vinculin (phospho Tyr100) antibody.
Dilution : 1:1000

WB analysis of COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA which were either untreated (Lane 1 and 6) or treated with vanadate for 24 hr (Lane 2-5 and 7-10) using GTX54517 Vinculin (phospho Tyr100) antibody (left panel) or Vinculin antibody (right panel).