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Vinculin (phospho Tyr1065) antibody

Anti-Vinculin (phospho Tyr1065) antibody used in Western Blot (WB). GTX54518
Anti-Vinculin (phospho Tyr1065) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54518

Cat No. GTX54518

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Chicken
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:250
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1mg/ml BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 1065. The sequence is conserved in mouse and chicken.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Vinculin (phospho Tyr1065) antibody used in Western Blot (WB). GTX54518

GTX54518 WB Image

WB analysis of COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA which were either untreated (Lane 1 and 6) or treated with vanadate for 24hr (Lane 2-5 and 7-10) using GTX54518 Vinculin (phospho Tyr1065) antibody (left panel) or Vinculin antibody (right panel).

Anti-Vinculin (phospho Tyr1065) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54518

GTX54518 ICC/IF Image

ICC/IF analysis of HUVEC cells using GTX54518 Vinculin (phospho Tyr1065) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379