WB analysis of COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA which were either untreated (Lane 1 and 6) or treated with vanadate for 24hr (Lane 2-5 and 7-10) using GTX54518 Vinculin (phospho Tyr1065) antibody (left panel) or Vinculin antibody (right panel).

ICC/IF analysis of HUVEC cells using GTX54518 Vinculin (phospho Tyr1065) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature