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Vinculin (phospho Tyr822) antibody

Orthogonal
Anti-Vinculin (phospho Tyr822) antibody used in IHC (Paraffin sections) (IHC-P). GTX54519
Anti-Vinculin (phospho Tyr822) antibody used in IHC (Paraffin sections) (IHC-P). GTX54519
Anti-Vinculin (phospho Tyr822) antibody used in Western Blot (WB). GTX54519

Cat. No. GTX54519

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Chicken
Package
100 μl ($399)
See all Vinculin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:10-1:100
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 822. The sequence is conserved in mouse, rat, and chicken.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vinculin

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton

Database

Research Area

DATA IMAGES

Anti-Vinculin (phospho Tyr822) antibody used in IHC (Paraffin sections) (IHC-P). GTX54519

GTX54519 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX54519 Vinculin (phospho Tyr822) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-Vinculin (phospho Tyr822) antibody used in IHC (Paraffin sections) (IHC-P). GTX54519

GTX54519 IHC-P Image

IHC-P analysis of rat heart tissue using GTX54519 Vinculin (phospho Tyr822) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-Vinculin (phospho Tyr822) antibody used in Western Blot (WB). GTX54519

GTX54519 WB Image

Orthogonal

WB (peptide competition) analysis of CEFs left untransfected (Lane 1) or transfected with Src (Lane 2-5) using GTX54519 Vinculin (phospho Tyr822) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399