GeneTex
United States (US)

Vinexin antibody [C1C3]

GTX115361_40597_WB_18121410_973.jpg
GTX115361_40597_IHC_18121410_159.jpg

Cat No. GTX115361

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

HepG2
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sorbin And Sh3 Domain Containing 3,Scam-1,Scam1,Sh3D4,Sorbs3

Cellular Localization

Isoform Alpha: Cell junction , Cytoplasm , cytoskeleton , Isoform Beta: Cell junction , Nucleus

Background

The SORBS3 gene encodes 2 isoforms vinexin-alpha and -beta that are vinculin (VCL; MIM 193065)-binding cytoskeletal proteins involved in focal adhesion and cell-cell adhesion (Kioka et al., 1999 [PubMed 9885244]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX115361_40597_WB_18121410_973.jpg

GTX115361 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX115361 diluted at 1:500

GTX115361_40597_IHC_18121410_159.jpg

GTX115361 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vinexin(GTX115361) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169