GeneTex
United States (US)

Vinexin antibody [N3C2], Internal

GTX115362_40597_IHC_18121410_133.jpg
GTX115362_40597_WB_18121410_131.jpg
GTX115362_40597_IFA_18121410_621.jpg

Cat No. GTX115362

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SCAM-1 antibody, SCAM1 antibody, SH3D4 antibody, VINEXIN antibody, SORBS3 antibody, vinexin beta (SH3-containing adaptor molecule-1) antibody, sorbin and SH3 domain-containing protein 3 antibody, sorbin and SH3 domain containing 3 antibody

Cellular Localization

Isoform Alpha: Cell junction (By similarity) , Cytoplasm , cytoskeleton (By similarity) , Isoform Beta: Cell junction (By similarity) , Nucleus

Background

The SORBS3 gene encodes 2 isoforms vinexin-alpha and -beta that are vinculin (VCL; MIM 193065)-binding cytoskeletal proteins involved in focal adhesion and cell-cell adhesion (Kioka et al., 1999 [PubMed 9885244]).[supplied by OMIM]
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Database

Research Area

DATA IMAGES
GTX115362_40597_IHC_18121410_133.jpg

GTX115362 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vinexin(GTX115362) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115362_40597_WB_18121410_131.jpg

GTX115362 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX115362 diluted at 1:2000

GTX115362_40597_IFA_18121410_621.jpg

GTX115362 ICC/IF Image

Vinexin antibody [N3C2], Internal detects SORBS3 protein at cytoskeleton by confocal immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SORBS3 protein stained by Vinexin antibody [N3C2], Internal (GTX115362) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Reference
SDS
Glycerol.pdf
Thimerosal.pdf