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Vinexin antibody [N3C2], Internal

KO/KD
Anti-Vinexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115362
Anti-Vinexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX115362
Anti-Vinexin antibody [N3C2], Internal used in Western Blot (WB). GTX115362
Anti-Vinexin antibody [N3C2], Internal used in Western Blot (WB). GTX115362
Anti-Vinexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115362
Anti-Vinexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115362

Cat. No. GTX115362

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all Vinexin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170568

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sorbin and SH3 domain containing 3 , SCAM-1 , SCAM1 , SH3D4

Cellular Localization

Isoform Alpha: Cell junction , Cytoplasm , cytoskeleton , Isoform Beta: Cell junction , Nucleus

Background

The SORBS3 gene encodes 2 isoforms vinexin-alpha and -beta that are vinculin (VCL; MIM 193065)-binding cytoskeletal proteins involved in focal adhesion and cell-cell adhesion (Kioka et al., 1999 [PubMed 9885244]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Vinexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115362

GTX115362 ICC/IF Image

Vinexin antibody [N3C2], Internal detects SORBS3 protein at cytoskeleton by confocal immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SORBS3 protein stained by Vinexin antibody [N3C2], Internal (GTX115362) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Anti-Vinexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX115362

GTX115362 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vinexin(GTX115362) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vinexin antibody [N3C2], Internal used in Western Blot (WB). GTX115362

GTX115362 WB Image

Various whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vinexin antibody [N3C2], Internal (GTX115362) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Vinexin antibody [N3C2], Internal used in Western Blot (WB). GTX115362

GTX115362 WB Image

KO/KD

The data was published in the journal PLoS One in 2014. PMID: 25237887

Anti-Vinexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115362

GTX115362 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 25237887

Anti-Vinexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115362

GTX115362 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 25237887

REFERENCE

Application Reference

Chang YW et al. Cell Cycle 2017; : 0 Midbody localization of Vinexin recruits Rhotekin to facilitate cytokinetic abscission.
Application : WB, ICC/IF
Reactivity : Human
Holle A.W. et al. Integr. Biol. 2016; 8 (10) : 1049-1058 High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation.
Application : ICC/IF
Reactivity : Human
Chang YW et al. PLoS One 2014; 9 (9) : e107961 Arsenite-Activated JNK Signaling Enhances CPEB4-Vinexin Interaction to Facilitate Stress Granule Assembly and Cell Survival.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169