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Visfatin antibody [4D5]

Anti-Visfatin antibody [4D5] used in Western Blot (WB). GTX50049

Cat No. GTX50049

Host

Mouse

Clonality

Monoclonal

Clone Name

4D5

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Visfatin (1-491aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Nicotinamide Phosphoribosyltransferase , 1110035O14Rik , Pbef , Pbef1 , Vf , Visfatin , Nampt

Background

Visfatin is pre-B cell colony-enhancing factor 1(PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.

Database

Research Area

DATA IMAGES

Anti-Visfatin antibody [4D5] used in Western Blot (WB). GTX50049

GTX50049 WB Image

Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

There are currently no references for Visfatin antibody [4D5] (GTX50049). Be the first to share your publications with this product.

REVIEW

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SDS
Sodium Azide.pdf