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Visfatin antibody [4D5]

Anti-Visfatin antibody [4D5] used in Western Blot (WB). GTX50049

Cat. No. GTX50049

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4D5

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Visfatin products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Visfatin (1-491aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nicotinamide phosphoribosyltransferase , 1110035O14Rik , PBEF , PBEF1 , VF , VISFATIN

Cellular Localization

Nucleus , Cytoplasm , Secreted

Background

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-Visfatin antibody [4D5] used in Western Blot (WB). GTX50049

GTX50049 WB Image

Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399