APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , Raji , Jurkat , K562 , THP-1 , HL-60 , NCI-H929 , A549 , HCT116 , MCF-7
Predict Reactivity
Mouse, Bovine, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Visfatin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10726139
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nicotinamide phosphoribosyltransferase , 1110035O14Rik , PBEF , PBEF1 , VF , VISFATIN
Cellular Localization
Cytoplasm
Background
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444](/templates/default/js/lightbox/images/loading.gif "Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444")
|
GTX117444 WB Image
Visfatin antibody detects Visfatin protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
Visfatin antibody (GTX117444) dilution: 1:1000
|
|
|
GTX117444 WB Image
Sample (30 ug of whole cell lysate)
A: A431
B: Raji
7.5% SDS PAGE
GTX117444 diluted at 1:1000
|
|
|
GTX117444 WB Image
Visfatin antibody detects Visfatin protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
C. 30 μg MCF-7 whole cell lysate/extract
7.5 % SDS-PAGE
Visfatin antibody (GTX117444) dilution: 1:1000
|
|
|
GTX117444 IHC-P Image
Visfatin antibody [N1N3] detects Visfatin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Visfatin antibody [N1N3] (GTX117444) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
There are currently no references for Visfatin antibody [N1N3] (GTX117444). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Visfatin antibody [N1N3] (GTX117444). Be the first to share your experience with this product.
![Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444](/upload/website/prouct_img/small/GTX117444/GTX117444_40478_WB_2_w_23060519_303.webp "Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444")
![Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444](/upload/website/prouct_img/small/GTX117444/GTX117444_40478_WB_w_23060519_556.webp "Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444")
![Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444](/upload/website/prouct_img/small/GTX117444/GTX117444_40478_WB_3_w_23060519_883.webp "Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444")
![Anti-Visfatin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX117444](/upload/website/prouct_img/small/GTX117444/GTX117444_40478_20160816_IHC-P_R_w_23060519_599.webp "Anti-Visfatin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX117444")
![Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444](/upload/website/prouct_img/normal/GTX117444/GTX117444_40478_WB_2_w_23060519_303.webp "Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444")
![Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444](/upload/website/prouct_img/normal/GTX117444/GTX117444_40478_WB_w_23060519_556.webp "Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444")
![Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444](/upload/website/prouct_img/normal/GTX117444/GTX117444_40478_WB_3_w_23060519_883.webp "Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444")
![Anti-Visfatin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX117444](/upload/website/prouct_img/normal/GTX117444/GTX117444_40478_20160816_IHC-P_R_w_23060519_599.webp "Anti-Visfatin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX117444")