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Visfatin antibody [N1N3]

Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444
Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444
Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444
Anti-Visfatin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX117444

Cat No. GTX117444

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , K562 , NCI-H929

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Visfatin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10726139

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nicotinamide phosphoribosyltransferase , 1110035O14Rik , PBEF , PBEF1 , VF , VISFATIN

Cellular Localization

Cytoplasm

Background

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444

GTX117444 WB Image

Visfatin antibody detects Visfatin protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
C. 30 μg MCF-7 whole cell lysate/extract
7.5 % SDS-PAGE
Visfatin antibody (GTX117444) dilution: 1:1000

Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444

GTX117444 WB Image

Visfatin antibody detects Visfatin protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
Visfatin antibody (GTX117444) dilution: 1:1000

Anti-Visfatin antibody [N1N3] used in Western Blot (WB). GTX117444

GTX117444 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: Raji
7.5% SDS PAGE
GTX117444 diluted at 1:1000

Anti-Visfatin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX117444

GTX117444 IHC-P Image

Visfatin antibody [N1N3] detects Visfatin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Visfatin antibody [N1N3] (GTX117444) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for Visfatin antibody [N1N3] (GTX117444). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169