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Visfatin antibody

Anti-Visfatin antibody used in IHC (Paraffin sections) (IHC-P). GTX128973
Anti-Visfatin antibody used in Immunoprecipitation (IP). GTX128973
Anti-Visfatin antibody used in Western Blot (WB). GTX128973
Anti-Visfatin antibody used in Western Blot (WB). GTX128973
Anti-Visfatin antibody used in Western Blot (WB). GTX128973
Anti-Visfatin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128973
Anti-Visfatin antibody used in Western Blot (WB). GTX128973

Cat No. GTX128973

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

NCI-H929 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , mouse muscle , Rat2

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Visfatin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nicotinamide phosphoribosyltransferase , 1110035O14Rik , PBEF , PBEF1 , VF , VISFATIN

Cellular Localization

Cytoplasm

Background

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Visfatin antibody used in IHC (Paraffin sections) (IHC-P). GTX128973

GTX128973 IHC-P Image

Visfatin antibody detects Visfatin protein at cytosol and nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Visfatin antibody (GTX128973) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Visfatin antibody used in Immunoprecipitation (IP). GTX128973

GTX128973 IP Image

Immunoprecipitation of Visfatin protein from 293T whole cell extracts using 5 μg of Visfatin antibody (GTX128973).
Western blot analysis was performed using Visfatin antibody (GTX128973).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Visfatin antibody used in Western Blot (WB). GTX128973

GTX128973 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Visfatin antibody (GTX128973) diluted at 1:500.

Anti-Visfatin antibody used in Western Blot (WB). GTX128973

GTX128973 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Visfatin antibody (GTX128973) diluted at 1:500.

Anti-Visfatin antibody used in Western Blot (WB). GTX128973

GTX128973 WB Image

NAMPT antibody detects Visfatin protein by western blot analysis.
A NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
Visfatin antibody (GTX128973) dilution: 1:1000

Anti-Visfatin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128973

GTX128973 ICC/IF Image

Visfatin antibody detects Visfatin protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in ice-cold MeOH for 5 min.
Green: Visfatin protein stained by Visfatin antibody (GTX128973) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Visfatin antibody used in Western Blot (WB). GTX128973

GTX128973 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Visfatin antibody (GTX128973) diluted at 1:500.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf