Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Visfatin antibody (GTX128980) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of Visfatin protein from 293T whole cell extracts using 5 μg of Visfatin antibody (GTX128980).
Western blot analysis was performed using Visfatin antibody (GTX128980).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunoprecipitation of Visfatin protein from 293T whole cell extracts using 5 μg of Visfatin antibody (GTX128980).
Western blot analysis was performed using Visfatin antibody (GTX128980).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Visfatin antibody (GTX128980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Visfatin antibody detects Visfatin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Visfatin protein stained by Visfatin antibody (GTX128980) diluted at 1:500.
Blue: Hoechst 33342 staining.

PBEF antibody detects Visfatin protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Visfatin antibody (GTX128980) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Visfatin antibody (GTX128980) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.