GeneTex
United States (US)

Visinin-like 1 antibody

GTX115039_WB_18121410_507.jpg
GTX115039_40247_20161012_IHC-Fr_R_18121410_794.jpg
GTX115039_40247_20161110_WB_M_R_18121410_763.jpg

Cat No. GTX115039

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:100000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

IMR32 , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Visinin-like 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HLP3 antibody, HPCAL3 antibody, HUVISL1 antibody, VILIP antibody, VILIP-1 antibody, VSNL1 antibody, hippocalcin-like protein 3 antibody, visinin-like protein 1 antibody, VLP-1 antibody, visinin-like 1 antibody

Background

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115039_WB_18121410_507.jpg

GTX115039 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX115039 diluted at 1:1000

GTX115039_40247_20161012_IHC-Fr_R_18121410_794.jpg

GTX115039 IHC-Fr Image

Visinin-like 1 antibody detects Visinin-like 1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Visinin-like 1 protein stained by Visinin-like 1 antibody (GTX115039) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX115039_40247_20161110_WB_M_R_18121410_763.jpg

GTX115039 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Visinin-like 1 antibody (GTX115039) diluted at 1:100000.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf