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Visinin-like 1 antibody

Anti-Visinin-like 1 antibody used in Western Blot (WB). GTX115039
Anti-Visinin-like 1 antibody used in Western Blot (WB). GTX115039
Anti-Visinin-like 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX115039
Anti-Visinin-like 1 antibody used in Western Blot (WB). GTX115039

Cat. No. GTX115039

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Rabbit
Package
100 μl ($399),
25 μl ($169)
See all Visinin-like 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:100000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

IMR32 , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Visinin-like 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620264

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

visinin like 1 , HLP3 , HPCAL3 , HUVISL1 , VILIP , VILIP-1

Background

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Visinin-like 1 antibody used in Western Blot (WB). GTX115039

GTX115039 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Visinin-like 1 antibody (GTX115039) diluted at 1:100000.

Anti-Visinin-like 1 antibody used in Western Blot (WB). GTX115039

GTX115039 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX115039 diluted at 1:1000

Anti-Visinin-like 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX115039

GTX115039 IHC-Fr Image

Visinin-like 1 antibody detects Visinin-like 1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Visinin-like 1 protein stained by Visinin-like 1 antibody (GTX115039) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Visinin-like 1 antibody used in Western Blot (WB). GTX115039

GTX115039 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28102204

REFERENCE

Application Reference

Dandan Liang et al. Nat Commun 2021; 12 (1) : 287 Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing.
Application : WB, IHC-P
Reactivity : Mouse, Rabbit
Wu VC et al. Sci Rep 2017; 7 : 39121 The prevalence of CTNNB1 mutations in primary aldosteronism and consequences for clinical outcomes.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169