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Vitamin D Binding Protein antibody

Anti-Vitamin D Binding Protein antibody used in Western Blot (WB). GTX109955
Anti-Vitamin D Binding Protein antibody used in Western Blot (WB). GTX109955
Anti-Vitamin D Binding Protein antibody used in IHC (Paraffin sections) (IHC-P). GTX109955
Anti-Vitamin D Binding Protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109955

Cat No. GTX109955

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HeLa , BCL-1

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vitamin D Binding Protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168148

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GC vitamin D binding protein , DBP , DBP-maf , DBP/GC , GRD3 , Gc-MAF , GcMAF , HEL-S-51 , VDB , VDBG , VDBP

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Vitamin D Binding Protein antibody used in Western Blot (WB). GTX109955

GTX109955 WB Image

Sample (30 μg of whole cell lysate)
A: BCL-1
10% SDS PAGE
GTX109955 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vitamin D Binding Protein antibody used in Western Blot (WB). GTX109955

GTX109955 WB Image

Vitamin D Binding Protein antibody detects GC protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
10% SDS-PAGE
Vitamin D Binding Protein antibody (GTX109955) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vitamin D Binding Protein antibody used in IHC (Paraffin sections) (IHC-P). GTX109955

GTX109955 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Vitamin D Binding Protein(GTX109955) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vitamin D Binding Protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109955

GTX109955 ICC/IF Image

Vitamin D Binding Protein antibody detects Vitamin D Binding Protein protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Vitamin D Binding Protein protein stained by Vitamin D Binding Protein antibody (GTX109955) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169