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Vitamin D Binding Protein antibody

Anti-Vitamin D Binding Protein antibody used in Western Blot (WB). GTX109955
Anti-Vitamin D Binding Protein antibody used in IHC (Paraffin sections) (IHC-P). GTX109955
Anti-Vitamin D Binding Protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109955
Anti-Vitamin D Binding Protein antibody used in Western Blot (WB). GTX109955

Cat. No. GTX109955

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Vitamin D Binding Protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HeLa , BCL-1

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vitamin D Binding Protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168148

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GC vitamin D binding protein , DBP , DBP-maf , DBP/GC , GRD3 , Gc-MAF , GcMAF , HEL-S-51 , VDB , VDBG , VDBP

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Vitamin D Binding Protein antibody used in Western Blot (WB). GTX109955

GTX109955 WB Image

Sample (30 μg of whole cell lysate)
A: BCL-1
10% SDS PAGE
GTX109955 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vitamin D Binding Protein antibody used in IHC (Paraffin sections) (IHC-P). GTX109955

GTX109955 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Vitamin D Binding Protein(GTX109955) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vitamin D Binding Protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109955

GTX109955 ICC/IF Image

Vitamin D Binding Protein antibody detects Vitamin D Binding Protein protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Vitamin D Binding Protein protein stained by Vitamin D Binding Protein antibody (GTX109955) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Vitamin D Binding Protein antibody used in Western Blot (WB). GTX109955

GTX109955 WB Image

Vitamin D Binding Protein antibody detects GC protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
10% SDS-PAGE
Vitamin D Binding Protein antibody (GTX109955) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169