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Vitronectin antibody

Orthogonal
Anti-Vitronectin antibody used in Western Blot (WB). GTX103475
Anti-Vitronectin antibody used in Western Blot (WB). GTX103475
Anti-Vitronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX103475
Anti-Vitronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX103475

Cat. No. GTX103475

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Vitronectin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

mouse brain

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vitronectin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169143

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vitronectin , V75 , VN , VNT

Cellular Localization

Secreted , extracellular space

Background

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Vitronectin antibody used in Western Blot (WB). GTX103475

GTX103475 WB Image

Vitronectin antibody detects Vitronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vitronectin antibody used in Western Blot (WB). GTX103475

GTX103475 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vitronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX103475

GTX103475 IHC-P Image

Vitronectin antibody detects Vitronectin protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Vitronectin stained by Vitronectin antibody (GTX103475) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vitronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX103475

GTX103475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vitronectin(GTX103475) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Candace R Fox et al. Viruses 2021; 14 (1) Complement Inhibitors Vitronectin and Clusterin Are Recruited from Human Serum to the Surface of Coronavirus OC43-Infected Lung Cells through Antibody-Dependent Mechanisms.
Application : WB, ICC/IF
Reactivity : Human
Sato K et al. Sci Rep 2021; 11 (1) : 5462 Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi.
Application : WB
Yang F et al. Cancer Res 2020; 80 (18) : 3892-3905 Interaction with CD68 and Regulation of GAS6 Expression by Endosialin in Fibroblasts Drives Recruitment and Polarization of Macrophages in Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Seto S et al. Front Microbiol 2019; 10 : 3081 Proteomic Profiling Reveals the Architecture of Granulomatous Lesions Caused by Tuberculosis and Mycobacterium avium Complex Lung Disease.
Application : IHC-P
Reactivity : Human
Lu CH et al. Arch Biochem Biophys 2013; 529 (2) : 146-56 Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.
Application : ELISA
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169