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Vitronectin antibody

Orthogonal
Anti-Vitronectin antibody used in Western Blot (WB). GTX103475
Anti-Vitronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX103475
Anti-Vitronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX103475
Anti-Vitronectin antibody used in Western Blot (WB). GTX103475

Cat No. GTX103475

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($379),
25 μl ($169)
See all Vitronectin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HepG2 conditioned medium , mouse brain

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vitronectin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169143

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vitronectin , V75 , VN , VNT

Cellular Localization

Secreted , extracellular space

Background

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Vitronectin antibody used in Western Blot (WB). GTX103475

GTX103475 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vitronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX103475

GTX103475 IHC-P Image

Vitronectin antibody detects Vitronectin protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Vitronectin stained by Vitronectin antibody (GTX103475) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vitronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX103475

GTX103475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vitronectin(GTX103475) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vitronectin antibody used in Western Blot (WB). GTX103475

GTX103475 WB Image

Vitronectin antibody detects Vitronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Candace R Fox et al. Viruses 2021; 14 (1) Complement Inhibitors Vitronectin and Clusterin Are Recruited from Human Serum to the Surface of Coronavirus OC43-Infected Lung Cells through Antibody-Dependent Mechanisms.
Sato K et al. Sci Rep 2021; 11 (1) : 5462 Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi.
Application : WB
Yang F et al. Cancer Res 2020; 80 (18) : 3892-3905 Interaction with CD68 and Regulation of GAS6 Expression by Endosialin in Fibroblasts Drives Recruitment and Polarization of Macrophages in Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Seto S et al. Front Microbiol 2019; 10 : 3081 Proteomic Profiling Reveals the Architecture of Granulomatous Lesions Caused by Tuberculosis and Mycobacterium avium Complex Lung Disease.
Application : IHC-P
Reactivity : Human
Lu CH et al. Arch Biochem Biophys 2013; 529 (2) : 146-56 Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.
Application : ELISA
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169