GeneTex
United States (US)

Vitronectin antibody

Orthogonal
GTX103475_40702_IHC_19011506_581.jpg
GTX103475_40702_20160121_WB_19011506_969.jpg
GTX103475_40702_WB_M_brain_19011506_600.jpg

Cat No. GTX103475

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HepG2 conditioned medium , mouse brain
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vitronectin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

V75 antibody, VN antibody, VNT antibody, VTN antibody, complement S-protein antibody, S-protein antibody, vitronectin antibody, epibolin antibody, serum-spreading factor antibody, serum spreading factor antibody, somatomedin B antibody

Cellular Localization

Secreted , extracellular space

Background

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103475_40702_IHC_19011506_581.jpg

GTX103475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vitronectin(GTX103475) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103475_40702_20160121_WB_19011506_969.jpg

GTX103475 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103475_40702_WB_M_brain_19011506_600.jpg

GTX103475 WB Image

Vitronectin antibody detects Vitronectin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169