Von Hippel Lindau antibody [3F391]

Cat No. GTX11189

Host	Mouse
Clonality	Monoclonal
Clone Name	3F391
Isotype	IgG1
Application	WB, ELISA, IHC
Reactivity	Human, Mouse
Package	250 μl ($359)

See all Von Hippel Lindau products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ELISA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Product Note

Does not recognize the truncated form of VHL protein.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.2% BSA, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein containing aa1-213 of human Von Hippel-Lindau Protein (vHL). Epitope: aa1-54.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_381272

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HRCA1 , RCA1 , VHL , VHL1 , pVHL , von HippelLindau tumor suppressor , Von Hippel Lindau , von Hippel-Lindau tumor suppressor

Cellular Localization

Cytoplasm,Membrane,Nucleus

Background

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]