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Von Hippel Lindau antibody, Internal

Anti-Von Hippel Lindau antibody, Internal used in ELISA (ELISA). GTX89268
Anti-Von Hippel Lindau antibody, Internal used in Western Blot (WB). GTX89268
Anti-Von Hippel Lindau antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89268

Cat No. GTX89268

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
IHC-P 5μg/ml
ELISA Assay dependent
Sandwich ELISA Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89268-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RSLVKPENYRRLD, from the internal region of the protein sequence according to NP_000542.1; NP_937799.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

von Hippel-Lindau tumor suppressor , HRCA1 , RCA1 , VHL1 , pVHL

Cellular Localization

Nucleus

Background

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Von Hippel Lindau antibody, Internal used in ELISA (ELISA). GTX89268

GTX89268 ELISA Image

ELISA analysis of Von Hippel Lindau protein using GTX89268 Von Hippel Lindau antibody, Internal.
Capture : GTX101087 (2.5μg/ml)
Detection : GTX89268 (1.5μg/ml)

Anti-Von Hippel Lindau antibody, Internal used in Western Blot (WB). GTX89268

GTX89268 WB Image

WB analysis of human ovary lysate using GTX89268 Von Hippel Lindau antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Von Hippel Lindau antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89268

GTX89268 IHC-P Image

IHC-P analysis of human kidney using GTX89268 Von Hippel Lindau antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf