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Von Hippel Lindau antibody

Orthogonal
GTX101087_39904_IHC_M_2_19011506_805.jpg
GTX101087_39904_IHC_19011506_468.jpg
GTX101087_39904_IHC_M_19011506_452.jpg
GTX101087_39904_20150410_IFA_19011506_390.jpg
GTX101087_39904_IFA_19011506_537.jpg
GTX101087_39904_IHC_R_19011506_725.jpg
GTX101087_43278_20180720_WB_B_19011506_195.jpg
GTX101087_43278_20181005_WB_19011506_863.jpg

Cat No. GTX101087

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

Myc-DDK-tagged VHL-transfected 293T

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VHL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HRCA1 antibody, RCA1 antibody, VHL1 antibody, VHL antibody, pVHL antibody, elongin binding protein antibody, protein G7 antibody, von Hippel-Lindau disease tumor suppressor antibody, von Hippel-Lindau tumor suppressor antibody

Cellular Localization

Isoform 1: Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Isoform 3: Cytoplasm

Background

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101087_39904_IHC_M_2_19011506_805.jpg

GTX101087 IHC-P Image

VHL antibody detects VHL protein at cytosol and nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
VHL antibody (GTX101087) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101087_39904_IHC_19011506_468.jpg

GTX101087 IHC-P Image

VHL antibody detects VHL protein at on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
VHL antibody (GTX101087) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101087_39904_IHC_M_19011506_452.jpg

GTX101087 IHC-P Image

VHL antibody detects VHL protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
VHL antibody (GTX101087) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101087_39904_20150410_IFA_19011506_390.jpg

GTX101087 ICC/IF Image

VHL antibody detects VHL protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VHL protein stained by VHL antibody (GTX101087) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.

GTX101087_39904_IFA_19011506_537.jpg

GTX101087 ICC/IF Image

VHL antibody detects VHL protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VHL protein stained by VHL antibody (GTX101087) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101087_39904_IHC_R_19011506_725.jpg

GTX101087 IHC-P Image

VHL antibody detects VHL protein at cytosol and nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
VHL antibody (GTX101087) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101087_43278_20180720_WB_B_19011506_195.jpg

GTX101087 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101087_43278_20181005_WB_19011506_863.jpg

GTX101087 WB Image

Orthogonal

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15240140

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf