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Von Hippel Lindau antibody

Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087
Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087
Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087
Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087
Anti-Von Hippel Lindau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101087

Cat No. GTX101087

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

Myc-DDK-tagged VHL-transfected 293T

Predict Reactivity

Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VHL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

von Hippel-Lindau tumor suppressor,HRCA1,RCA1,VHL1,pVHL

Cellular Localization

Isoform 1: Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Isoform 3: Cytoplasm

Background

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087

GTX101087 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087

GTX101087 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 15240140

Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

GTX101087 IHC-P Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

GTX101087 IHC-P Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Von Hippel Lindau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101087

GTX101087 ICC/IF Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169