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WAVE1 antibody

Anti-WAVE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133263
Anti-WAVE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133263
Anti-WAVE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133263
Anti-WAVE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133263
Anti-WAVE1 antibody used in Western Blot (WB). GTX133263

Cat. No. GTX133263

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all WAVE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse WAVE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886896

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WAS protein family, member 1 , AI195380 , AI838537 , Scar , WAVE , WAVE-1

Cellular Localization

Cytoplasm , cytoskeleton

Background

Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.

Database

Research Area

DATA IMAGES

Anti-WAVE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133263

GTX133263 ICC/IF Image

WAVE1 antibody detects WAVE1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WAVE1 protein stained by WAVE1 antibody (GTX133263) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-WAVE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133263

GTX133263 IHC-P Image

WAVE1 antibody detects WAVE1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
WAVE1 antibody (GTX133263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WAVE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133263

GTX133263 IHC-P Image

WAVE1 antibody detects WAVE1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
WAVE1 antibody (GTX133263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WAVE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133263

GTX133263 ICC/IF Image

WAVE1 antibody detects WAVE1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WAVE1 stained by WAVE1 antibody (GTX133263) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-WAVE1 antibody used in Western Blot (WB). GTX133263

GTX133263 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WAVE1 antibody (GTX133263) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169