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WAVE1 (phospho Tyr125) antibody

Anti-WAVE1 (phospho Tyr125) antibody used in IHC (Paraffin sections) (IHC-P). GTX55451
Anti-WAVE1 (phospho Tyr125) antibody used in Western Blot (WB). GTX55451

Cat. No. GTX55451

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all WAVE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 125 (E-T-Y(p)-D-V) derived from human WAVE1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WASP family member 1 , SCAR1 , WAVE , WAVE1

Cellular Localization

Cytoplasm, cytoskeleton,Cell junction, synapse

Background

The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-WAVE1 (phospho Tyr125) antibody used in IHC (Paraffin sections) (IHC-P). GTX55451

GTX55451 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX55451 WAVE1 (phospho Tyr125) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-WAVE1 (phospho Tyr125) antibody used in Western Blot (WB). GTX55451

GTX55451 WB Image

WB analysis of extracts from NIH/3T3 cells treated with Insulin (0.01U/ml 15mins) using GTX55451 WAVE1 (phospho Tyr125) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399