APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
2-10 ug/ml |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , human NT-2 , mouse brain extract
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human WBP11
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
WW domain binding protein 11 , NPWBP , PPP1R165 , SIPP1 , WBP-11
Cellular Localization
Nucleus,Cytoplasm
Background
This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX46466 IHC-P Image
IHC-P analysis of human pineal tissue using GTX46466 WBP11 antibody at 1:100. Left to right : DAPI, WBP11, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.
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GTX46466 WB Image
WB analysis of HeLa cells using GTX46466 WBP11 antibody at 0.2-1μg/ml.
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GTX46466 IP Image
IP analysis of mouse brain tissue using GTX46466 WBP11 antibody at 1:500. IP antibody: 6μg.
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GTX46466 WB Image
WB analysis of human NT-2 cells and mouse brain tissue extract using GTX46466 WBP11 antibody at 2μg/ml.
Lane 1 : human NT-2 cells
Lane 2 : mouse brain tissue
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REFERENCE
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REVIEW
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