GeneTex

  • Country / Location Selection

United States (US)

WBP11 antibody

Anti-WBP11 antibody used in Immunoprecipitation (IP). GTX118654
Anti-WBP11 antibody used in Western Blot (WB). GTX118654
Anti-WBP11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118654
Anti-WBP11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118654
Anti-WBP11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118654
Anti-WBP11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118654

Cat. No. GTX118654

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all WBP11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Jurkat , Raji

Predict Reactivity

Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WBP11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162007

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WW domain binding protein 11 , NPWBP , PPP1R165 , SIPP1 , WBP-11

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-WBP11 antibody used in Immunoprecipitation (IP). GTX118654

GTX118654 IP Image

WBP11 antibody immunoprecipitates WBP11 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of WBP11 protein by 4 μg WBP11 antibody (GTX118654)
7.5 % SDS-PAGE
The immunoprecipitated WBP11 protein was detected by WBP11 antibody (GTX118654) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-WBP11 antibody used in Western Blot (WB). GTX118654

GTX118654 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WBP11 antibody (GTX118654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-WBP11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118654

GTX118654 IHC-P Image

WBP11 antibody detects WBP11 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
WBP11 antibody (GTX118654) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WBP11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118654

GTX118654 IHC-P Image

WBP11 antibody detects WBP11 protein at nucleus in rat adrenal by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal.
WBP11 antibody (GTX118654) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WBP11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118654

GTX118654 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using WBP11(GTX118654) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WBP11 antibody used in IHC (Paraffin sections) (IHC-P). GTX118654

GTX118654 IHC-P Image

WBP11 antibody detects WBP11 protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
WBP11 antibody (GTX118654) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for WBP11 antibody (GTX118654). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for WBP11 antibody (GTX118654). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169