WBSCR22 antibody

Name: WBSCR22 antibody
Brand: GeneTex
SKU: GTX105840
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-WBSCR22 antibody used in Immunoprecipitation (IP). GTX105840

Anti-WBSCR22 antibody used in IHC (Paraffin sections) (IHC-P). GTX105840

Anti-WBSCR22 antibody used in Western Blot (WB). GTX105840

Anti-WBSCR22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105840

Cat. No. GTX105840

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all WBSCR22 products

View product citations for antibody GTX105840 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:500-1:1000

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLa , HCT116

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WBSCR22. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952564

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUD23 rRNA methyltransferase and ribosome maturation factor , HASJ4442 , HUSSY-3 , MERM1 , PP3381 , WBMT , WBSCR22

Cellular Localization

Nucleus

Background

This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. [provided by RefSeq]

Database

Gene ID: 114049 BUD23
UniProt: O43709 BUD23

Research Area

DATA IMAGES

GTX105840 IP Image

WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 μg of WBSCR22 antibody (GTX105840) 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody (GTX105840) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105840 IHC-P Image

WBSCR22 antibody detects WBSCR22 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WBSCR22 antibody (GTX105840) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105840 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using WBSCR22 (GTX105840) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105840 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HCT116
12% SDS PAGE
GTX105840 diluted at 1:1000

GTX105840 ICC/IF Image

WBSCR22 antibody detects WBSCR22 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WBSCR22 protein stained by WBSCR22 antibody (GTX105840) diluted at 1:200.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Zhao H et al. Biochem Genet 2020; (Epub) WBSCR22 Competes with Long Non-coding RNA Linc00346 for miR-509-5p Binding Site to Regulate Cancer Stem Cell Phenotypes of Colorectal Cancer.

Application : WB

Reactivity : Human

Baxter M et al. Elife 2020; 9 Cardiac mitochondrial function depends on BUD23 mediated ribosome programming.

Application : WB

Reactivity : Human

Zhao H et al. Am J Cancer Res 2018; 8 (5) : 824-834 natural killer cells inhibit *-resistant colorectal cancer by repressing wbscr22 via upregulating microrna-146b-5p.

Application : WB

Reactivity : Human

Nakazawa Y et al. Cancer Res 2011; 71 (3) : 1146-55 The novel metastasis promoter Merm1/Wbscr22 enhances tumor cell survival in the vasculature by suppressing Zac1/p53-dependent apoptosis.

Application : ICC/IF

Reactivity : Human

Submit a Reference

REVIEW