GeneTex
United States (US)

WBSCR22 antibody

GTX105840_40632_IP_19011506_853.jpg
GTX105840_39757_WB_19011506_318.jpg
GTX105840_IHC_19011506_576.jpg
GTX105840_39757_20150206_IHC_M_19011506_547.jpg
GTX105840_39757_20150410_IFA_19011506_375.jpg

Cat No. GTX105840

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLa , HCT116 , *A375M ,

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WBSCR22. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ44236 antibody, HASJ4442 antibody, HUSSY-3 antibody, MGC19709 antibody, MGC2022 antibody, MGC5140 antibody, PP3381 antibody, WBMT antibody, WBSCR22 antibody, Williams-Beuren candidate region putative methyltransferase antibody, Williams-Beuren syndrome chromosomal region 22 protein antibody, uncharacterized methyltransferase WBSCR22 antibody, Williams Beuren syndrome chromosome region 22 antibody

Cellular Localization

Nucleus

Background

This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. [provided by RefSeq]

Database

DATA IMAGES
GTX105840_40632_IP_19011506_853.jpg

GTX105840 IP Image

WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 μg of WBSCR22 antibody (GTX105840) 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody (GTX105840) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105840_39757_WB_19011506_318.jpg

GTX105840 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HCT116
12% SDS PAGE
GTX105840 diluted at 1:1000

GTX105840_IHC_19011506_576.jpg

GTX105840 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using WBSCR22 (GTX105840) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105840_39757_20150206_IHC_M_19011506_547.jpg

GTX105840 IHC-P Image

WBSCR22 antibody detects WBSCR22 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WBSCR22 antibody (GTX105840) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105840_39757_20150410_IFA_19011506_375.jpg

GTX105840 ICC/IF Image

WBSCR22 antibody detects WBSCR22 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WBSCR22 protein stained by WBSCR22 antibody (GTX105840) diluted at 1:200.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

WBSCR22 antibody Cat No. GTX105840

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 12-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A375M cells
Amount used : 26μg
Blocking : 1% skim milk /TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169