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WDR1 antibody

Anti-WDR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102214
Anti-WDR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102214
Anti-WDR1 antibody used in Western Blot (WB). GTX102214
Anti-WDR1 antibody used in Western Blot (WB). GTX102214

Cat No. GTX102214

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

Raji , mouse brain

Predict Reactivity

Rat, Bovine, Dog, Chicken, Guinea pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human WDR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

WD repeat domain 1 , AIP1 , HEL-S-52 , NORI-1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
Anti-WDR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102214

GTX102214 IHC-P Image

WDR1 antibody detects WDR1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
WDR1 antibody (GTX102214) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WDR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102214

GTX102214 IHC-P Image

WDR1 antibody detects WDR1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
WDR1 antibody (GTX102214) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WDR1 antibody used in Western Blot (WB). GTX102214

GTX102214 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102214 diluted at 1:5000

Anti-WDR1 antibody used in Western Blot (WB). GTX102214

GTX102214 WB Image

Sample(30 μg of whole cell lysate)
A:Raji (GTX27908)
7.5% SDS PAGE
GTX102214 diluted at 1:500

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169