GeneTex
United States (US)

WDR1 antibody

GTX102214_39911_IHC_M_19011506_844.jpg
GTX102214_39911_IHC_19011506_809.jpg
GTX102214_39911_WB_M_brain_19011506_286.jpg
GTX102214_WB_19011506_593.jpg

Cat No. GTX102214

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

Raji , mouse brain

Predict Reactivity

Rat, Bovine, Dog, Chicken, Guinea pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human WDR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AIP1 antibody, NORI-1 antibody, WDR1 antibody, WD repeat-containing protein 1 antibody, actin-interacting protein 1 antibody, WD repeat domain 1 antibody

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX102214_39911_IHC_M_19011506_844.jpg

GTX102214 IHC-P Image

WDR1 antibody detects WDR1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
WDR1 antibody (GTX102214) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102214_39911_IHC_19011506_809.jpg

GTX102214 IHC-P Image

WDR1 antibody detects WDR1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
WDR1 antibody (GTX102214) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102214_39911_WB_M_brain_19011506_286.jpg

GTX102214 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102214 diluted at 1:5000

GTX102214_WB_19011506_593.jpg

GTX102214 WB Image

Sample(30 μg of whole cell lysate)
A:Raji (GTX27908)
7.5% SDS PAGE
GTX102214 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169