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WDR19 antibody [N1N2], N-term

Anti-WDR19 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119202
Anti-WDR19 antibody [N1N2], N-term used in Western Blot (WB). GTX119202
Anti-WDR19 antibody [N1N2], N-term used in Western Blot (WB). GTX119202
Anti-WDR19 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119202

Cat No. GTX119202

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDR19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617362

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

WD repeat domain 19 , ATD5 , CED4 , DYF-2 , IFT144 , NPHP13 , ORF26 , Oseg6 , PWDMP , SRTD5

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains six WD repeats, a clathrin heavy-chain repeat, and three transmembrane domains. This gene is conserved from C. elegans to human. It may participate in androgen-regulated signaling mechanisms or in the vesicular trafficking of androgen-regulated secretory processes. Alternatively spliced transcript variants encoding distinct isoforms have been reported but the full-length nature of one of these variants has not been defined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-WDR19 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119202

GTX119202 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using WDR19(GTX119202) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WDR19 antibody [N1N2], N-term used in Western Blot (WB). GTX119202

GTX119202 WB Image

WDR19 antibody detects WDR19 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WDR19 antibody (GTX119202) diluted by 1:1000.

Anti-WDR19 antibody [N1N2], N-term used in Western Blot (WB). GTX119202

GTX119202 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX119202 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-WDR19 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119202

GTX119202 IHC-P Image

WDR19 antibody [N1N2], N-term detects WDR19 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
WDR19 antibody [N1N2], N-term (GTX119202) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169