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WDR61 antibody

Anti-WDR61 antibody used in Western Blot (WB). GTX116881
Anti-WDR61 antibody used in Western Blot (WB). GTX116881
Anti-WDR61 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116881

Cat. No. GTX116881

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all WDR61 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HepG2 , Mouse stomach

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDR61. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615372

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WD repeat domain 61 , REC14 , SKI8

Background

WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-WDR61 antibody used in Western Blot (WB). GTX116881

GTX116881 WB Image

Sample (50 ug of whole cell lysate)
A: mouse stomach
12% SDS PAGE
GTX116881 diluted at 1:1000

Anti-WDR61 antibody used in Western Blot (WB). GTX116881

GTX116881 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX116881 diluted at 1:500

Anti-WDR61 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116881

GTX116881 ICC/IF Image

WDR61 antibody detects WDR61 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: WDR61 protein stained by WDR61 antibody (GTX116881) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169