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WDR77 antibody [N2C3]

Anti-WDR77 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116802
Anti-WDR77 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116802
Anti-WDR77 antibody [N2C3] used in Western Blot (WB). GTX116802

Cat. No. GTX116802

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all WDR77 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDR77. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615368

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WD repeat domain 77 , HKMT1069 , MEP-50 , MEP50 , Nbla10071 , p44 , p44/Mep50

Cellular Localization

Nucleus , Cytoplasm

Background

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-WDR77 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116802

GTX116802 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using WDR77(GTX116802) antibody at 1:200 dilution.

Anti-WDR77 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116802

GTX116802 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using WDR77(GTX116802) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WDR77 antibody [N2C3] used in Western Blot (WB). GTX116802

GTX116802 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX116802 diluted at 1:10000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169