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WDSUB1 antibody

Anti-WDSUB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116382
Anti-WDSUB1 antibody used in Western Blot (WB). GTX116382
Anti-WDSUB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX116382

Cat. No. GTX116382

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all WDSUB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDSUB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617686

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WD repeat, sterile alpha motif and U-box domain containing 1 , UBOX6 , WDSAM1

Database

Research Area

DATA IMAGES

Anti-WDSUB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116382

GTX116382 ICC/IF Image

WDSUB1 antibody detects WDSUB1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WDSUB1 protein stained by WDSUB1 antibody (GTX116382) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-WDSUB1 antibody used in Western Blot (WB). GTX116382

GTX116382 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX116382 diluted at 1:2000
Anti-WDSUB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX116382

GTX116382 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using WDSUB1(GTX116382) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169