Immunohistochemical analysis of paraffin-embedded human breast cancer, using WDSUB1(GTX116382) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX116382 diluted at 1:2000

WDSUB1 antibody detects WDSUB1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WDSUB1 protein stained by WDSUB1 antibody (GTX116382) diluted at 1:500.
Blue: Hoechst 33342 staining.