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WDYHV1 antibody

Anti-WDYHV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120298
Anti-WDYHV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120298
Anti-WDYHV1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120298

Cat No. GTX120298

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDYHV1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885479

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

WDYHV motif containing 1 , C8orf32

Background

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.

Database

DATA IMAGES

Anti-WDYHV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120298

GTX120298 IHC-P Image

WDYHV1 antibody detects WDYHV1 protein at nucleus in human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human breast cancer.
WDYHV1 antibody (GTX120298) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WDYHV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120298

GTX120298 IHC-P Image

WDYHV1 antibody detects WDYHV1 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
WDYHV1 antibody (GTX120298) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WDYHV1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120298

GTX120298 ICC/IF Image

WDYHV1 antibody detects WDYHV1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WDYHV1 protein stained by WDYHV1 antibody (GTX120298) diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169