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WEE1 antibody [N3C2], Internal

Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392
Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392
Anti-WEE1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111392
Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392

Cat No. GTX111392

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Observed MW

95 kDa. The observed M.W. is based on the following publication. PMID: 24766806 and 23051732

Positive Control

Jurkat , Raji , NCI-H929 , Neuro2A , C8D30 , C2C12

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WEE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038242

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

WEE1 G2 checkpoint kinase , WEE1A , WEE1hu

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392

GTX111392 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24766806 and 23051732

Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392

GTX111392 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-WEE1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111392

GTX111392 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 Xenograft, using WEE1(GTX111392) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392

GTX111392 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:1000 and competitor's antibody (CST#13084) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169