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WEE1 antibody [N3C2], Internal

Comparison
Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392
Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392
Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392
Anti-WEE1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111392

Cat. No. GTX111392

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all WEE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Observed MW

95 kDa. The observed M.W. is based on the following publication. PMID: 24766806 and 23051732

Positive Control

Neuro2A , C8D30 , C2C12 , Jurkat , Raji , NCI-H929

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WEE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038242

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WEE1 G2 checkpoint kinase , WEE1A , WEE1hu

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392

GTX111392 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24766806 and 23051732

Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392

GTX111392 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-WEE1 antibody [N3C2], Internal used in Western Blot (WB). GTX111392

GTX111392 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:1000 and competitor's antibody (CST#13084) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-WEE1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111392

GTX111392 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 Xenograft, using WEE1(GTX111392) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

CP Chen et al. Biomed Pharmacother 2023; 166 : 115389
Application : WB
Senese S et al. Mol Biol Cell 2015; 26 (3) : 440-52 A unique insertion in STARD9's motor domain regulates its stability.
Application : WB
Reactivity : Human
Cheung AK et al. Mol Cancer Ther 2013; 12 (8) : 1393-401 Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169