APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 4-6μg/ml |
|
Note :
IHC-P
Human Kidney shows nuclear staining, stronger in DCT compared to PCT.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89707-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_064520.2) and isoform 2 (NP_569079.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence EELRGVDFFKQRRC, from the C Terminus of the protein sequence according to NP_064520.2; NP_569079.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Werner helicase interacting protein 1 , WHIP , bA420G6.2
Cellular Localization
Nucleus , Cytoplasm
Background
Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus. It has sequence similarity to replication factor C family proteins and is conserved from E. coli to human. This protein likely accumulates at sites of DNA damage by interacting with polyubiquinated proteins and also binds to DNA polymerase delta and increases the initiation frequency of DNA polymerase delta-mediated DNA synthesis. This protein also interacts with nucleoporins at nuclear pore complexes. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq, Jul 2012]
Database
Research Area
DATA IMAGES
|
GTX89707 IHC-P Image
IHC-P analysis of human kidney using GTX89707 WHIP antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 4μg/ml
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GTX89707 WB Image
WB analysis of Jurkat lysate using GTX89707 WHIP antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for WHIP antibody, C-term (GTX89707). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for WHIP antibody, C-term (GTX89707). Be the first to share your experience with this product.
