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WHIP antibody

Anti-WHIP antibody used in Western Blot (WB). GTX24731

Cat. No. GTX24731

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Monkey
Package
50 μg ($399)
See all WHIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ELISA 1:10000 - 1:40000
IHC Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: SRESYDAPPTPSGAC conjugated to KLH, corresponding to amino acids 107-120 of Human WHIP.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_423851

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Werner helicase interacting protein 1 , WHIP , bA420G6.2

Cellular Localization

Nucleus,Cytoplasm

Background

WHIP Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein containing the exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene may influence the aging process.

Database

Research Area

DATA IMAGES

Anti-WHIP antibody used in Western Blot (WB). GTX24731

GTX24731 WB Image

Western blot analysis is shown using GeneTex Affinity Purified anti-Human WHIP antibody (GTX24731) to detect Human WHIP present in a HEK293 whole cell lysate. ~30mg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using infrared labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1:5,000 for 45 min. The fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.

REFERENCE

Application Reference

Crosetto N et al. J Biol Chem 2008; 283 (50) : 35173-85 Human Wrnip1 is localized in replication factories in a ubiquitin-binding zinc finger-dependent manner.
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399