WB analysis of 293T whole cell lysate using GTX24731 WHIP antibody.
Loading : 30 μg
Dilution : 1:200

WB analysis of 293T whole cell lysate using GTX24731 WHIP antibody prior incubated with (Lane B) or without (Lane A) blocking peptide.
Loading : 30 μg
Dilution : 1:1000

Western blot analysis is shown using GeneTex Affinity Purified anti-Human WHIP antibody (GTX24731) to detect Human WHIP present in a HEK293 whole cell lysate. ~30mg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using infrared labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1:5,000 for 45 min. The fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.