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WIF1 antibody [1G5]

Anti-WIF1 antibody [1G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82765
Anti-WIF1 antibody [1G5] used in Western Blot (WB). GTX82765
Anti-WIF1 antibody [1G5] used in IHC (Paraffin sections) (IHC-P). GTX82765

Cat. No. GTX82765

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1G5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all WIF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human WIF1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11165730

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WNT inhibitory factor 1 , WIF-1

Cellular Localization

Secreted

Background

The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-WIF1 antibody [1G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82765

GTX82765 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82765 WIF1 antibody [1G5].
Green : WIF1
Red: Actin filaments

Anti-WIF1 antibody [1G5] used in Western Blot (WB). GTX82765

GTX82765 WB Image

WB analysis of HeLa (1), NIH3T3 (2) and NTERA-2 (3) cell lysate using GTX82765 WIF1 antibody [1G5].

Anti-WIF1 antibody [1G5] used in IHC (Paraffin sections) (IHC-P). GTX82765

GTX82765 IHC-P Image

IHC-P analysis of ovary tumour tissue (left) and lung cancer (right) using GTX82765 WIF1 antibody [1G5].

REFERENCE

Application Reference

Zhang K et al. Mol Cancer 2018; 17 (1) : 153 Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399