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WIPI1 antibody

Overexpression
Anti-WIPI1 antibody used in Western Blot (WB). GTX128167
Anti-WIPI1 antibody used in Western Blot (WB). GTX128167

Cat. No. GTX128167

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all WIPI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

mouse muscle , human WIPI1-transfected 293T cells

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human WIPI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885721

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WD repeat domain, phosphoinositide interacting 1 , ATG18 , ATG18A , WIPI49

Cellular Localization

Golgi apparatus , trans-Golgi network , Endosome , Cytoplasmic vesicle , clathrin-coated vesicle

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-WIPI1 antibody used in Western Blot (WB). GTX128167

GTX128167 WB Image

Overexpression

WIPI1 antibody detects WIPI1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human WIPI1-transfected 293T cells
10 % SDS-PAGE
WIPI1 antibody (GTX128167) dilution: 1:10000

Anti-WIPI1 antibody used in Western Blot (WB). GTX128167

GTX128167 WB Image

WIPI1 antibody detects WIPI1 protein by Western blot analysis.
A. 50 μg mouse muscle lysate/extract
10 % SDS-PAGE
WIPI1 antibody (GTX128167) dilution: 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169