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WIPI1 antibody

Anti-WIPI1 antibody used in Western Blot (WB). GTX128167
Anti-WIPI1 antibody used in Western Blot (WB). GTX128167

Cat No. GTX128167

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

human WIPI1-transfected 293T cells , mouse muscle

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human WIPI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

WD repeat domain, phosphoinositide interacting 1 , ATG18 , ATG18A , WIPI49

Cellular Localization

Golgi apparatus , trans-Golgi network , Endosome , Cytoplasmic vesicle , clathrin-coated vesicle

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-WIPI1 antibody used in Western Blot (WB). GTX128167

GTX128167 WB Image

WIPI1 antibody detects WIPI1 protein by Western blot analysis.
A. 50 μg mouse muscle lysate/extract
10 % SDS-PAGE
WIPI1 antibody (GTX128167) dilution: 1:500

Anti-WIPI1 antibody used in Western Blot (WB). GTX128167

GTX128167 WB Image

WIPI1 antibody detects WIPI1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human WIPI1-transfected 293T cells
10 % SDS-PAGE
WIPI1 antibody (GTX128167) dilution: 1:10000

REFERENCE

There are currently no references for WIPI1 antibody (GTX128167). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf