*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HepG2 , human WIPI1-transfected 293T
Mouse, Rat, Bovine, Pig(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the C-terminus region of human WIPI1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
WD repeat domain, phosphoinositide interacting 1 , ATG18 , ATG18A , WIPI49
Golgi apparatus , trans-Golgi network , Endosome , Cytoplasmic vesicle , clathrin-coated vesicle
WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]
GTX129279 WB Image
WIPI1 antibody detects WIPI1 protein by western blot analysis. Non-transfected (-) and -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WIPI1 antibody (GTX129279) diluted by 1:20000.
GTX129279 WB Image
WIPI1 antibody detects WIPI1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with WIPI1 antibody (GTX129279) diluted by 1:500