APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:20000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 , human WIPI1-transfected 293T cells
Predict Reactivity
Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human WIPI1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885952
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
WD repeat domain, phosphoinositide interacting 1 , ATG18 , ATG18A , WIPI49
Cellular Localization
Golgi apparatus , trans-Golgi network , Endosome , Cytoplasmic vesicle , clathrin-coated vesicle
Background
WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX129279 WB Image
WIPI1 antibody detects WIPI1 protein by western blot analysis. Non-transfected (-) and -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WIPI1 antibody (GTX129279) diluted by 1:20000.
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GTX129279 WB Image
WIPI1 antibody detects WIPI1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with WIPI1 antibody (GTX129279) diluted by 1:500
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REFERENCE
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REVIEW
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