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WIPI1 antibody

Anti-WIPI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31497
Anti-WIPI1 antibody used in Western Blot (WB). GTX31497

Cat. No. GTX31497

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all WIPI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Rat Colon Tissue Lysate(GTX24034)

Product Note

At least two isoforms of WIPI1 are known to exist; this antibody will detect both isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

WIPI1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WIPI1.The immunogen is located within amino acids 350 - 400 of WIPI1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WD repeat domain, phosphoinositide interacting 1 , ATG18 , ATG18A , WIPI49

Cellular Localization

Endosome,Cytoplasm, cytoskeleton

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-WIPI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31497

GTX31497 IHC-P Image

IHC-P analysis of human colon tissue using GTX31497 WIPI1 antibody.
Working concentration : 20 μg/ml

Anti-WIPI1 antibody used in Western Blot (WB). GTX31497

GTX31497 WB Image

WB analysis of rat colon tissue lysate using GTX31497 WIPI1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399