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WIPI2 antibody

Anti-WIPI2 antibody used in Western Blot (WB). GTX132453
Anti-WIPI2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132453
Anti-WIPI2 antibody used in Western Blot (WB). GTX132453
Anti-WIPI2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132453

Cat No. GTX132453

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($329),
25 μl ($169)
See all WIPI2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

K562 , THP-1 , Jurkat

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human WIPI2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

WD repeat domain, phosphoinositide interacting 2 , ATG18B , Atg21 , CGI-50 , WIPI-2

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-WIPI2 antibody used in Western Blot (WB). GTX132453

GTX132453 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WIPI2 antibody (GTX132453) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-WIPI2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132453

GTX132453 ICC/IF Image

WIPI2 antibody detects WIPI2 protein at Preautophagosomal structure membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: WIPI2 protein stained by WIPI2 antibody (GTX132453) diluted at 1:2500.
Blue: Hoechst 33342 staining.
Anti-WIPI2 antibody used in Western Blot (WB). GTX132453

GTX132453 WB Image

Various whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with WIPI2 antibody (GTX132453) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-WIPI2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132453

GTX132453 IHC-P Image

WIPI2 antibody detects WIPI2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
WIPI2 antibody (GTX132453) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169