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WNK1 antibody [HL2184]

Recombinant antibody Orthogonal
Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188
Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188
Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188
Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188
Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188
Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188

Cat. No. GTX638188

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2184

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all WNK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

251 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human WNK1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WNK lysine deficient protein kinase 1 , HSAN2 , HSN2 , KDP , PPP1R167 , PRKWNK1 , PSK , p65

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188

GTX638188 IHC-P Image

WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
WNK1 stained by WNK1 antibody [HL2184] (GTX638188) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188

GTX638188 IHC-P Image

WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WNK1 stained by WNK1 antibody [HL2184] (GTX638188) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188

GTX638188 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (GTX638188) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188

GTX638188 ICC/IF Image

WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WNK1 stained by WNK1 antibody [HL2184] (GTX638188) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188

GTX638188 ICC/IF Image

WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WNK1 stained by WNK1 antibody [HL2184] (GTX638188) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188

GTX638188 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (GTX638188) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations