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WNK1 antibody

Anti-WNK1 antibody used in Western Blot (WB). GTX106197
Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197
Anti-WNK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106197
Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197
Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197

Cat No. GTX106197

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

251 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Rat, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038244

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

WNK lysine deficient protein kinase 1 , HSAN2 , HSN2 , KDP , PPP1R167 , PRKWNK1 , PSK , p65

Cellular Localization

Cytoplasm

Background

The WNK1 gene encodes a cytoplasmic serine-threonine kinase expressed in distal nephron.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-WNK1 antibody used in Western Blot (WB). GTX106197

GTX106197 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody (GTX106197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197

GTX106197 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using WNK1(GTX106197) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WNK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106197

GTX106197 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using WNK1(GTX106197) antibody at 1:200 dilution.

Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197

GTX106197 IHC-P Image

WNK1 antibody detects WNK1 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
WNK1 antibody (GTX106197) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197

GTX106197 IHC-P Image

WNK1 antibody detects WNK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WNK1 stained by WNK1 antibody (GTX106197) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169