GeneTex
United States (US)

WNK1 antibody

GTX106197_WB_19011506_259.jpg
GTX106197_39764_IHC_19011506_363.jpg
GTX106197_39764_IFA_19011506_813.jpg
GTX106197_39764_20151223_IHC-P_19011506_576.jpg

Cat No. GTX106197

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

251 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Rat, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HSAN2 antibody, HSN2 antibody, KDP antibody, KIAA0344 antibody, MGC163339 antibody, MGC163341 antibody, PRKWNK1 antibody, PSK antibody, p65 antibody, WNK1 antibody, prostate-derived sterile 20-like kinase antibody, protein kinase with no lysine 1 antibody, serine/threonine-protein kinase WNK1 antibody, erythrocyte 65 kDa protein antibody, WNK lysine deficient protein kinase 1 antibody

Cellular Localization

Cytoplasm

Background

The WNK1 gene encodes a cytoplasmic serine-threonine kinase expressed in distal nephron.[supplied by OMIM]

Database

DATA IMAGES
GTX106197_WB_19011506_259.jpg

GTX106197 WB Image

Sample (30 µg of whole cell lysate)
A: H1299
5% SDS PAGE
GTX106197 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106197_39764_IHC_19011506_363.jpg

GTX106197 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using WNK1(GTX106197) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106197_39764_IFA_19011506_813.jpg

GTX106197 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using WNK1(GTX106197) antibody at 1:200 dilution.

GTX106197_39764_20151223_IHC-P_19011506_576.jpg

GTX106197 IHC-P Image

WNK1 antibody detects WNK1 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
WNK1 antibody (GTX106197) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169