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WNK1 antibody

Anti-WNK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32973
Anti-WNK1 antibody used in Western Blot (WB). GTX32973

Cat. No. GTX32973

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all WNK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

251 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human WNK1 (NP_055638.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887690

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WNK lysine deficient protein kinase 1 , HSAN2 , HSN2 , KDP , PPP1R167 , PRKWNK1 , PSK , p65

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-WNK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32973

GTX32973 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32973 WNK1 antibody.
Blue : DAPI

Anti-WNK1 antibody used in Western Blot (WB). GTX32973

GTX32973 WB Image

WB analysis of various sample lysates using GTX32973 WNK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Ding L et al. J Biol Chem 2017; (Epub) Akt3 Kinase Suppresses Pinocytosis of Low-Density-Lipoprotein By Macrophages via Novel WNK/SGK1/Cdc42 Pathway.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399